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Dimethylglutarate (DMGL) is an endogenous compound with various and complex metabolic pathways. In this study, we examined whether DMGL may directly influence mitochondrial respiration and glycolysis in human fibroblasts. The resting state of oxygen consumption and of lactate generation in fibroblasts was not affected by DMGL, but the effect of DMGL on stimulated oxygen consumption was dose-dependent and reached statistical significance at DMGL concentrations of 5 mM. Glycolytic capacity of fibroblasts was stimulated by DMGL in a dose-dependent manner with a significant effect at concentrations of 3 and 5 mM. In addition, the DMGL effect was reversible and washed out when the cells were washed twice with phosphate-buffered saline. The effect of DMGL was completely abolished by removing the medium from the incubator, and was completely reversible by adding glucose back. These findings are in agreement with the notion that DMGL may serve as a metabolic intermediate to conserve glucose for cell proliferation.

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